During the course the student will get acquainted with the basic concepts and principles of general and applied microbiology, including virology. They become familiar with the structure of prokaryotic and eukaryotic cells. Students will be informed about the principle of growth and multiplication of microorganisms and the influence of physical and chemical factors on microbial cells. Another aim of the subject will be an explanation of differences in the metabolism of photosynthetic, chemoautotrophic and heterotrophic microorganisms. Emphasis will be placed on clarifying the importance of microorganisms as pathogens and, on the contrary, their importance in the industry and in the environment. Course graduates will also learn about the most important eukaryotic microorganisms and their representatives.
Course graduates will be able to:
- Understand and correctly apply the basic concepts and principles of general and applied microbiology, including virology;
- to distinguish and compare prokaryotes and eukaryotes, to compare archaea and bacteria;
- to list the properties and to explain the functions of the structures present in the bacterial cell, the archaeal cell and the cell of eukaryotic microorganisms;
- to distinguish photosynthetic, chemoatotrophic and heterotrophic microorganisms according to how they acquire energy;
- to demonstrate the importance of bacteria as pathogens and to describe the importance of selected microorganisms in industry and in the environment;
1. Introduction to microbiology - recommended literature, assessment methods and criteria, history of microbiology. 2. Bacteria - cell structure and function, bacterial motility and endospores. 3. Microbial growth - growth curve, continuous cultivation of bacteria, influences of environmental factors on the growth. 4. Bacterial nutrition and metabolism - requirements for carbon, nitrogen, sulphur, phosphorus, growth factors, uptake of nutrients, introduction to metabolism. 5. Bacterial catabolism - chemoorganotrophic processes - aerobic and anaerobic respiration,fermentation, catabolism of carbohydrates, lipids and proteins, chemolithotrophy and phototrophy. Anabolism - principles, CO2 fixation, synthesis of sugars and polysaccharides, lipids, amino acids, purines, pyrimidines and nucleotides. Regulation of metabolism. 6. Bacterial genetics - chromosome, plasmids, gene expression, replication, transcription, translation, mechanisms of genetic variation. 7. Bacterial diseases, microbiology of food, applied environmental microbiology. 8. Cyanobacteria - cell structure, metabolism, ecology, industrial applications. 9. Archaea - cell structure and function, extremely halophilic and extremely thermophilic archaea, methanogenic archaea. 10. Yeasts - cell structure, metabolism, reproduction,yeasts as pathogens, industrial applications. 11. Fungi - fungal structure and function, reproduction and life cycles, common members of this group. 12. Viruses and other acellular agents - structure, viral multiplication, types of viral infections, cultivation of viruses, prions.
NĚMEC, Miroslav and Dagmar MATOULKOVÁ. Základy obecné mikrobiologie (Introduction to General Microbiology). 1. vyd. Brno: Masarykova univerzita, 2015. 256 pp. ISBN 978-80-210-7923-6. info
MADIGAN, Michael T. Brock biology of microorganisms. 12th ed. San Francisco, Calif.: Pearson/Benjamin Cummings, 2009. xxviii, 10. ISBN 9780132324601. info
NĚMEC, Miroslav and Dana HORÁKOVÁ. Základy mikrobiologie pro učitelské studium. 2. vyd. Brno: Masarykova univerzita v Brně, 1993. 233 s. ISBN 8021008172. info
KAPRÁLEK, František. Základy bakteriologie. Praha: Karolinum, 2000. 241 s. ISBN 8071848115. info
ŠILHÁNKOVÁ, Ludmila. Mikrobiologie pro potravináře a biotechnology. Vyd. 3., opr. a dopl., v Aca. Praha: Academia, 2002. 363 s. ISBN 8020010246. info
VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1. vyd. Brno: Neptun, 2001. 247 pp. ISBN 80-902896-2-2. info
WILLEY, Joanne M., Linda SHERWOOD and Christopher J. WOOLVERTON. Prescott's principles of microbiology. Edited by Lansing M. Prescott. 1st ed. Boston: McGraw-Hill Higher Education, 2009. xiv, 847. ISBN 9780071283670. info
Lecture - Power Point presentation
Final assessment is based on written examination. Final exam will be written multiple choice test consisting of 30 questions evaluated by 30 points. 17 points is needed to pass the final exam.