PřF:Bi7900 Genetic methods in zoology - Course Information
Bi7900 Genetic methods in zoologyFaculty of Science
- Extent and Intensity
- 2/1/0. 3 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- prof. RNDr. Miloš Macholán, CSc. (lecturer)
prof. Mgr. et Mgr. Josef Bryja, Ph.D. (lecturer)
- Guaranteed by
- prof. RNDr. Miloš Macholán, CSc.
Department of Botany and Zoology - Biology Section - Faculty of Science
Contact Person: prof. Mgr. et Mgr. Josef Bryja, Ph.D.
Supplier department: Department of Botany and Zoology - Biology Section - Faculty of Science
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Course objectives
- The aim of this course is to give undergraduate and PhD students a basic orientation in principles of morphological, cytogenetic, and molecular genetics methods currently used in zoological and evolutionary research. After finishing the course, students should gain theoretical knowledge and practical skills in the following topics: Analysis of phenotype (epigenetic traits, quantitative traits, geometric morphometrics); Cytogenetics (karyotype analysis, banding, FISH, "chromosome painting“); Genotyping of DNA markers (DNA extraction, PCR, real-time PCR, Sanger sequencing, fingerprinting, RAPD, AFLP, analysis of microsatellites, LINE, SINE, and SNP genotyping - RFLP, DGGE, TGEE, SSCP, cloning, etc.); High-throughput genotyping (microarrays, transcriptomics, next generation sequencing); Basic methods of data analysis (description of genetic variability, work with genetic databases - BLAST, Dryad, etc.).
- Learning outcomes
- Student will be able to select and apply specific genetic technique for solving various questions in ecology, systematics or evolutionary biology.
- 1. Introduction (importance of genetic methods in zoology and evolutionary biology; overview of genetic approaches, etc.)
- 2. Analysis of phenotype (signal phenotypes, epigenetic traits, quantitative traits, landmark analysis)
- 3. Cytogenetics (karyotype analysis, banding, FISH, "chromosome painting") and electrophoresis of allozymes
- 4. DNA analysis I (DNA extraction, genetic markers - nuclear vs. cytoplasmic DNA, PCR, real-time PCR, Sanger sequencing)
- 5. DNA analysis II ("single-locus" DNA markers: microsatellites, LINE, SINE)
- 6. DNA analysis III (SNP and their analysis: RFLP, DGGE, TGEE, SSCP, cloning, new methods of SNP genotyping - SNP chips, etc.)
- 7. DNA analysis IV ("multilocus" DNA markers: fingerprinting, RAPD, AFLP)
- 8. DNA analysis V (next generation sequencing)
- 9. Analysis of gene expression (microarrays, qPCR, transcriptomics)
- 10. Basic analysis of genetic data (data formats, aligning of sequences, working with genetic databases - GenBank, NCBI, BLAST, Dryad, etc.)
- Laboratory training (in one block) - DNA extraction, PCR, qPCR, DNA electrophoresis, genotyping of microsatellites, Sanger sequencing, NGS genotyping of amplicons
- ZIMA, Jan. Genetické metody v zoologii. 1. vyd. Praha: Karolinum, 2004. 239 s. ISBN 8024607956. info
- Teaching methods
- lectures, practical classes
- Assessment methods
- lectures and laboratory courses; Each student will take an oral exam including at least 3-4 short inquiries from various areas specified in the syllabus. Emphasized are questions closely related to research activities of the student.
- Language of instruction
- Follow-Up Courses
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Listed among pre-requisites of other courses
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/spring2022/Bi7900