Bi7900 Genetic methods in zoology

Faculty of Science
Spring 2022
Extent and Intensity
2/1/0. 3 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
prof. RNDr. Miloš Macholán, CSc. (lecturer)
prof. Mgr. et Mgr. Josef Bryja, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Miloš Macholán, CSc.
Department of Botany and Zoology - Biology Section - Faculty of Science
Contact Person: prof. Mgr. et Mgr. Josef Bryja, Ph.D.
Supplier department: Department of Botany and Zoology - Biology Section - Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this course is to give undergraduate and PhD students a basic orientation in principles of morphological, cytogenetic, and molecular genetics methods currently used in zoological and evolutionary research. After finishing the course, students should gain theoretical knowledge and practical skills in the following topics: Analysis of phenotype (epigenetic traits, quantitative traits, geometric morphometrics); Cytogenetics (karyotype analysis, banding, FISH, "chromosome painting“); Genotyping of DNA markers (DNA extraction, PCR, real-time PCR, Sanger sequencing, fingerprinting, RAPD, AFLP, analysis of microsatellites, LINE, SINE, and SNP genotyping - RFLP, DGGE, TGEE, SSCP, cloning, etc.); High-throughput genotyping (microarrays, transcriptomics, next generation sequencing); Basic methods of data analysis (description of genetic variability, work with genetic databases - BLAST, Dryad, etc.).
Learning outcomes
Student will be able to select and apply specific genetic technique for solving various questions in ecology, systematics or evolutionary biology.
  • 1. Introduction (importance of genetic methods in zoology and evolutionary biology; overview of genetic approaches, etc.)
  • 2. Analysis of phenotype (signal phenotypes, epigenetic traits, quantitative traits, landmark analysis)
  • 3. Cytogenetics (karyotype analysis, banding, FISH, "chromosome painting") and electrophoresis of allozymes
  • 4. DNA analysis I (DNA extraction, genetic markers - nuclear vs. cytoplasmic DNA, PCR, real-time PCR, Sanger sequencing)
  • 5. DNA analysis II ("single-locus" DNA markers: microsatellites, LINE, SINE)
  • 6. DNA analysis III (SNP and their analysis: RFLP, DGGE, TGEE, SSCP, cloning, new methods of SNP genotyping - SNP chips, etc.)
  • 7. DNA analysis IV ("multilocus" DNA markers: fingerprinting, RAPD, AFLP)
  • 8. DNA analysis V (next generation sequencing)
  • 9. Analysis of gene expression (microarrays, qPCR, transcriptomics)
  • 10. Basic analysis of genetic data (data formats, aligning of sequences, working with genetic databases - GenBank, NCBI, BLAST, Dryad, etc.)
  • Laboratory training (in one block) - DNA extraction, PCR, qPCR, DNA electrophoresis, genotyping of microsatellites, Sanger sequencing, NGS genotyping of amplicons
  • ZIMA, Jan. Genetické metody v zoologii. 1. vyd. Praha: Karolinum, 2004. 239 s. ISBN 8024607956. info
Teaching methods
lectures, practical classes
Assessment methods
lectures and laboratory courses; Each student will take an oral exam including at least 3-4 short inquiries from various areas specified in the syllabus. Emphasized are questions closely related to research activities of the student.
Language of instruction
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021.
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