CS

PřF:Bi5599 App.Chem.and Biochem. - Course Information

Bi5599 Applied Chemistry and Biochemistry

Faculty of Science
Autumn 2008
Extent and Intensity
2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Jan Vondráček, Ph.D. (lecturer)
RNDr. Miroslav Machala, CSc. (lecturer), MVDr. Mgr. Monika Dušková, Ph.D. (deputy)
Supervisor
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Jan Vondráček, Ph.D.
Timetable
Thu 9:00–10:50 BFU
Prerequisites (in Czech)
Bi4020 Molecular biology && C3580 Biochemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Main objectives of the course are to provide students with an overview of basic methods for analysis of macromolecules (DNA, RNA, proteins, lipids); with laboratory techniques for work with low molecular weight compounds; analyses of cell populations and subcellular structures; basic principles of in vivo experiments employing genetically modified mammals. The course is taught as lecture and seminar aimed at work with primary literature, in order to understand the importance of individual techniques and to interpret both literary and experimental data. Students should actively participate in seminars.
Syllabus
  • 1)Protein analysis; separation techniques; Western blotting, protein-protein interactions; protein localization; protein post-translational modifications; proteomics; manipulations of protein expression.
  • 2)Basic immunochemistry techniques.
  • 3)DNA isolation, separation; PCR techniques, plasmid work, transfection of eukaryotic cells.
  • 4)RNA isolation, detection (Northern blotting, RT-PCR), microarrays, siRNA.
  • 5)Lipids and polysacharidy; isolation, separation and identification.
  • 6)Low-molecular-weight compounds - separation and analytical techniques; HPLC techniques and their modifications.
  • 7)Specific cellular assays.
  • 8)Fluorescennce techniques.
  • 9)Aplication of in vivo mammalian models in physiology.
Literature
  • Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
  • Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
  • Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000
Assessment methods
Final seminar. Each student is required to prepare a presentation about methodical approaches used in an assigned paper.
Language of instruction
Czech
Further Comments
Study Materials
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018.
  • Enrolment Statistics (Autumn 2008, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2008/Bi5599