PřF:Bi5599 App.Chem.and Biochem. - Course Information
Bi5599 Applied Chemistry and BiochemistryFaculty of Science
- Extent and Intensity
- 2/0. 2 credit(s) (plus extra credits for completion). Type of Completion: graded credit.
- prof. RNDr. Jan Vondráček, Ph.D. (lecturer)
- prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Jan Vondráček, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- Mon 9:00–10:50 A11/333
- Prerequisites (in Czech)
- Bi4020 Molecular biology && C3580 Biochemistry
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- Fields of study the course is directly associated with
- Course objectives
- At the end of the course students should be able to understand the importance of individual chemical/biochemical techniques and to interpret both their own experimental data, based on the techniques taught in the course. They should be able to work with the information in the primary literature, in order to understand and implement the techniques described in primary scientific literature in the area of animal physiology and molecular physiology. They should make reasoned decisions about the importance of the data and to interpret their biological importance. Based on this, they will be able to present and explain the techniques used in their own work to expert audience.
- 1)Protein analysis; separation techniques; Western blotting, protein-protein interactions; protein localization; protein post-translational modifications; proteomics; manipulations of protein expression.
- 2)Basic immunochemistry techniques.
- 3)DNA isolation, separation; PCR techniques, plasmid work, transfection of eukaryotic cells.
- 4)RNA isolation, detection (Northern blotting, RT-PCR), microarrays, siRNA.
- 5)Lipids and polysaccharides; isolation, separation and identification.
- 6)Low-molecular-weight compounds - separation and analytical techniques; HPLC techniques and their modifications.
- 7)Specific cellular assays.
- 8)Fluorescence techniques.
- 9)Application of in vivo mammalian models in physiology.
- Wilson et al.: Principles and Techniques of Practical Biochemistry, 5th ed., CUP, 2000
- Klouda, Moderní analytické metody, Pavel Klouda, Ostrava, 2003
- Alberts et al.: Molecular Biology of the Cell, 4th ed., Garland Science, 2002
- Teaching methods
- lectures, class discussion
- Assessment methods
- The course is finished as colloquium. Each student is required to prepare a short presentation (10 minutes) about methodical approaches used in an assigned scientific paper. Students summarize in their presentation both foundations and results of the assigned paper, and tehy commentin deatil the methodology used - a specific method is described by them according to the selection made by lecturers. Final evaluation is based on the quality of presentation and on the outcome of discussion with lecturers.
- Language of instruction
- Further comments (probably available only in Czech)
- Study Materials
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
- Listed among pre-requisites of other courses