PřF:Bi8241 Plant genetics practice - Course Information
Bi8241 Plant genetics practice
Faculty of ScienceSpring 2011
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Jana Řepková, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Jana Řepková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jana Řepková, CSc. - Timetable
- Wed 8:00–11:50 C13/318
- Prerequisites
- ( Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B3060 Basic genetics || Bi3060 Basic genetics ) && ( Ex_3065 Molekulární biologie || Imp_9115 Molekulární biologie || B3120 Molecular and cell biology || B4030 Molecular biology || B5740 Molecular biology || B6130 Molecular biology || B7940 Molecular biology || B4020 Molecular biology || Bi4035 Molecular biology - lab.pract. )
Mastering of requirements of B3060 and B4020 are convenient. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16 - fields of study / plans the course is directly associated with
- Ecotoxicology (programme PřF, M-BI)
- Ecotoxicology (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- General Biology (programme PřF, M-BI, specialization Plant Physiology)
- General Biology (programme PřF, N-BI, specialization Plant Physiology)
- Course objectives
- The practical course of plant genetics is aimed at practical application of genetic and molecular-genetic methods in plants: mutant and transgenic plants analysis, plant DNA isolation, transgene detection by PCR, molecular markers in genetic mapping. Completion of this course will ensure to the student to obtain the practical experiences with the particular laboratory methods.
- Syllabus
- 1. Screening of transgenic plants, 2. Medium with antibiotics preparation, 3. Mutant phenotypes screening, 4. Plant DNA isolation, 5. Transgen identification by means of PCR, 6. Molecular markers - SSR, CAPS, 7. Genetic mapping by means of molecular markers, 8. Plant databases.
- Literature
- ŘEPKOVÁ, Jana and Jiřina RELICHOVÁ. Genetika rostlin (Plant Genetics). 1st ed. Brno: Masarykova univerzita Brno, 2001, 269 pp. 3489/Př-12/01-17/30. ISBN 80-210-2736-3. info
- WEIGEL, Detlef and Jane GLAZEBROOK. Arabidopsis : a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 2002, xii, 354 s. ISBN 0-87969-572-2. info
- Teaching methods
- Practical courses 4 hours long every two weeks. Protocols from individual topics.
- Assessment methods
- Three final group projects.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
- Enrolment Statistics (Spring 2011, recent)
- Permalink: https://is.muni.cz/course/sci/spring2011/Bi8241