ZLBC041c Biochemistry II - practice

Faculty of Medicine
Spring 2009
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
PharmDr. Lenka Adámková, Ph.D. (seminar tutor)
Mgr. Jan Alán (seminar tutor)
RNDr. Hana Bochořáková (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
Mgr. Kateřina Chvátalová, Ph.D. (seminar tutor)
Mgr. Martin Klepárník, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
Mgr. Milena Matejovičová, Ph.D. (seminar tutor)
Ing. Jitka Melounová, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Lenka Nerudová (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
ZLBC041c/21: each even Thursday 7:30–9:20 A16/211
ZLBC041c/22: each even Wednesday 7:30–9:20 A16/211
ZLBC041c/23: each even Thursday 7:30–9:20 A16/211
ZLBC041c/24: each even Wednesday 7:30–9:20 A16/211
ZLBC041c/40: No timetable has been entered into IS.
Prerequisites (in Czech)
ZLLC011p Med.Chem.-lect. && ZLBF011p Med.Physics and Informat.-lect && ZLBI0222p Biology II - lecture && ZLBC0221s Biochemistry I - seminar && ZLFY0321c Physiol. I - pract. && ZLFY0321s Physiol. I - sem.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to VSLC0421p and VSLC0421s. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics
Syllabus
  • Handling biological material. Drawing blood specimens. 1.2 Blood serum. Anticoagulants, blood plasma. 1.3 Biochemical analyzers. 1.4 Interpretation of test results. 1.5 Reporting of test results Laboratory investigations of lipid and cholesterol metabolism 2.1 The enzymatic assay of total serum cholesterol. 2.2 Determination of serum triacylglycerols. 2.3 HDL-cholesterol determination, calculation of the LDL-cholesterol. 2.4 The chylomicron test. 2.5 Electrophoresis of lipoproteins. Investigations of saccharide metabolism. Diabetes mellitus 3.1 The enzymatic assay of glucose in blood. 3.2 Estimation of blood glucose using a personal glucometer. 3.3 The oral glucose tolerance test (oGTT). 3.4 Detection and determination of glucose in urine. 3.5 Detection of ketone bodies in urine. 3.6 Determination of glycated haemoglobin (HbA1) in blood. 3.7 The long-term complications of diabetes – microalbuminuria. Selected investigations in liver diseases 4.1 Determination of alanine aminotransferase (ALT) activity. 4.2 Determination of aspartate aminotransferase (AST) activity. 5.2 Detection of bilirubin in urine. 5.3 Detection of urobilinogens in urine. Laboratory diagnostics of myocardial infarction 6.1 Measurement of total plasma creatine kinase activity. 6.2 Measurement of plasma CK-MB activity. 6.3 Measurement of plasma troponin T concentration. 6.4 Measurement of plasma myoglobin concentration. Investigations of plasma proteins 7.1 Determination of total serum proteins by the biuret method. 7.2 Determination of blood serum albumin. 7.3 Electrophoresis of serum proteins. 7.4 Assay for increased level of C-reactive protein. 7.5 Determination of IgE in blood serum (ELISA). 7.6 Determination of ferritin in blood serum (ELISA). Compensatory practicals. Credits.
Literature
  • TÁBORSKÁ, Eva, Josef TOMANDL and Hana PAULOVÁ. Biochemie. 3. přepracované vydání. Brno: Masarykova univerzita, 2008, 126 stran. ISBN 9788021045163. info
  • Biochemistry. Edited by Josef Tomandl - Eva Táborská. 1st ed. Brno: Masaryk University, 2008, 81 s. ISBN 9788021045477. info
Assessment methods
Practicals in biochemistry lab.
Full attendance in all lessons (making up all missing and justified lessons), completion of all lab reports and handing them to the teacher till the given data. Obtaining of course-unit credits of practices is the pre-requisite for registration to the examination of Biochemistry I.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: mimo studenty LF, max 5 posluchačů z MU, po dohodě s vedoucím ústavu
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/med/spring2009/ZLBC041c