Bi7430 Molecular biotechnology

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Taught in person.
prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
Mgr. David Kovář, Ph.D. (lecturer)
Ing. RNDr. Martin Marek, Ph.D. (lecturer)
Stanislav Mazurenko, PhD (lecturer)
Mgr. Šárka Nevolová, Ph.D. (lecturer)
Mgr. Pavel Dvořák, Ph.D. (lecturer)
Mgr. Martina Damborská (assistant)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Wed 11:00–12:50 B11/305
Basic knowledge of biochemistry, microbiology, molecular biology and genetics.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to provide comprehensive information on the molecular basis and major applications of recombinant DNA technology with an emphasis on environmentally friendly processes meeting modern trends of sustainable development.

The methodological part of the course provides a broad multidisciplinary overview of the fields of molecular biology, modern techniques of recombinant protein expression, synthetic biology, microfluidics and the use of artificial intelligence in the field.

The second part of the lectures focuses on the practical applications of recombinant DNA technology in the chemical and pharmaceutical industries, medicine, agriculture, and environmental protection.

Graduates will have a theoretical background and a comprehensive overview of the field relevant to their future employment in research laboratories, biomedical research, and development or the biotechnology industry.
Learning outcomes
The student will have an up-to-date overview of the advanced methods used in the field of molecular biotechnology, particularly:
- molecular biology and modification of DNA
- modern protein expression technologies
- basic methods in synthetic biology
- protein engineering
- microfluidics and "lab on chip" technologies
- artificial intelligence in life sciences

During the course the student will also get an up-to-date overview of modern molecular biotechnology applications in the following areas:
- medicine and advanced diagnostics
- long-term sustainable industries
- modern agriculture and biofuels
- environmental protection

At the end of this course, the student will understand the molecular nature of modern biotechnology and the use of recombinant DNA technology in industrial, agricultural, pharmaceutical and biomedical applications. Students will have the theoretical foundation and a comprehensive overview of the field important for their future career in research laboratories, biomedical research, and development or biotechnology industry.
  • Introduction to Molecular Biotechnology
  • Advanced Molecular Biology Tools
  • Protein Expression Technologies
  • Introduction to Synthetic Biology
  • Protein Engineering
  • Microfludics - Lab on a Chip
  • Artificial Intelligence in Life Sciences
  • Molecular Biotechnology Industry
  • Molecular Biotechnology in Medicine I.
  • Molecular Biotechnology in Medicine II.
  • Molecular Biotechnology in Agriculture
  • Environmental Molecular Biotechnology
  • (the order of courses can be changed)
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011. xxxiii, 60. ISBN 9783527326372. info
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010. xvi, 1000. ISBN 9781555814984. info
  • Molecular biology and biotechnology. Edited by John M. Walker - Ralph Rapley. 5th ed. Cambridge: Royal Society of Chemistry, 2009. xix, 604. ISBN 9780854041251. info
Teaching methods
During lecturing, the PowerPoint presentations are used, PDF materials for printing and notices are available before each course, short written tests at the beginning of selected lectures and short exercises and discussions during lectures are prepared. For each course, there is recommended literature on the topic (eg current publications, reviews, books chapters ).
Assessment methods
Short written tests will be prepared at the beginning of selected lectures. Each short written test will contain 10-12 questions on the previous curriculum. Final evaluation of the course will be based on the cumulative results of these short written tests.

In the case that (i) the student will not fulfill all short tests, (ii) will not be successful in the short tests, or (iii) is not satisfied with the result of the short testing, he or she will be evaluated by the general written test. Terms for the general written test will be listed in the current examination period, the test will contain 50 questions from overall course content, the time to complete the test will be 1 hour.

The student receives a score according to the following criteria (success in test questions / evaluation: 80% or more /A; 65-79% /B; 50-64% /C; 40-49% /D; 25-39% /E; less than 25% /F.
Language of instruction
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020.
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