PřF:B6400 Methods of molecular biology

B6400 Methods of molecular biology

Faculty of Science
Spring 2000

Extent and Intensity

2/0/0. 2 credit(s). Type of Completion: zk (examination).

Teacher(s)

Guaranteed by

Prerequisites (in Czech)

B4020 Molecular biology || B4030 Molecular biology || B6130 Molecular biology

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Syllabus

Basic techniques in molecular biology. Centrifugation techniques. Electrophoretic and electronmicroscopic methods for DNA analysis. In vitro manipulation with DNA. Enzymes in molecular biology. Isolation, identification and characterization of DNA fragments. Restriction analysis of DNA, physical mapping of genomes. DNA sequencing, genomic sequencing, analysis of DNA sequences. Hybridization techniques, preparation of hybridization probes. Principle of DNA cloning, cloning strategy. Main types of prokaryotic vectors. Gene and genomic libraries. Identification of recombinant DNA, recombinant DNA selection and screening. Expression in E. coli of cloned DNA molecules. Electrophoresis of proteins. Western blotting. Flow cytometry. Polymerase chain reaction. Transcription and translation in vitro.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

Bi8090 Gene engineering
Ex_2979 || Ex_2980 || B6390 || B6400 || Bi6400 || NOW(Bi6400) || souhlas
Bi8313 Genetic Engineering - Laboratory Course
!B6492 && (Ex_2979 || Ex_2980 || B6390 || B6400 || Bi6400) && (Bi6405 || B6405 || Bi6400c) && NOW(Bi8090) && !Bi5491

The course is also listed under the following terms Spring 2001, Spring 2002.