Bi6270 Cytogenetics and cytogenomics
Faculty of ScienceSpring 2025
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
RNDr. Vladimíra Vallová, Ph.D. (lecturer) - Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ) || Bi4010 Essential molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic and cytogenomics techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Learning outcomes
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Genetics, cytogenetics and cytogenomics. Human cytogenetics. 2. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, fluorescent microscopy. 3. Basic techniques of cytogenetic preparation making. 4. Mitosis, meiosis and cell division, chromosomal structure and organization of chromosomes. 5. Methods of clinical cytogenetics, classical cytogenetics, mitotic metaphase chromosome preparation, karyotype, idiogram. Chromosome banding methods: Q, G, C, R, Ag -banding. Automation of metaphase finding and imaging. An International Systém of Human Cytogenomics Nomenclature (ISCN2016). 6. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in clinical and cancer cytogenetics. 7. Interphase FISH, multicolor FISH (M-FISH), spectral karyotyping (SKY), mBAND. Microarray-CGH. NGS in cytogenetics. 8. Clinical cytogenetics and cytogenomics. Cytogenetics in reproduction medicine. Prenatal and preimplantation cytogenetic diagnosis, detection of common aneuploidies. 9. Mechanisms of chromosome rearrangements, chromosome structural variation, copy number variation (CNVs). 10. The origin and consequences of chromosome pathology, microdeletion and microduplication syndromes, inversion, translocation. Marker chromosomes. 11. Cytogenetic abnormalities in hematological malignancies and solid tumors, chromothripsis. 12. Application of short-term cytogenetics assays for screening of environmental mutagens – CAPL test, micronucleus test, sister chromatid exchanges. 13. The European standard EN ISO 15189:2013 Requirements for quality and competence of medical (cytogenetic) laboratories.
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 % of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics and cytogenomics
Faculty of ScienceSpring 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
RNDr. Vladimíra Vallová, Ph.D. (lecturer) - Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 19. 2. to Sun 26. 5. Wed 8:00–9:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ) || Bi4010 Essential molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic and cytogenomics techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Learning outcomes
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Genetics, cytogenetics and cytogenomics. Human cytogenetics. 2. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, fluorescent microscopy. 3. Basic techniques of cytogenetic preparation making. 4. Mitosis, meiosis and cell division, chromosomal structure and organization of chromosomes. 5. Methods of clinical cytogenetics, classical cytogenetics, mitotic metaphase chromosome preparation, karyotype, idiogram. Chromosome banding methods: Q, G, C, R, Ag -banding. Automation of metaphase finding and imaging. An International Systém of Human Cytogenomics Nomenclature (ISCN2016). 6. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in clinical and cancer cytogenetics. 7. Interphase FISH, multicolor FISH (M-FISH), spectral karyotyping (SKY), mBAND. Microarray-CGH. NGS in cytogenetics. 8. Clinical cytogenetics and cytogenomics. Cytogenetics in reproduction medicine. Prenatal and preimplantation cytogenetic diagnosis, detection of common aneuploidies. 9. Mechanisms of chromosome rearrangements, chromosome structural variation, copy number variation (CNVs). 10. The origin and consequences of chromosome pathology, microdeletion and microduplication syndromes, inversion, translocation. Marker chromosomes. 11. Cytogenetic abnormalities in hematological malignancies and solid tumors, chromothripsis. 12. Application of short-term cytogenetics assays for screening of environmental mutagens – CAPL test, micronucleus test, sister chromatid exchanges. 13. The European standard EN ISO 15189:2013 Requirements for quality and competence of medical (cytogenetic) laboratories.
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 % of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics and cytogenomics
Faculty of ScienceSpring 2023
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 8:00–9:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ) || Bi4010 Essential molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic and cytogenomics techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Learning outcomes
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Genetics, cytogenetics and cytogenomics. Human cytogenetics. 2. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, fluorescent microscopy. 3. Basic techniques of cytogenetic preparation making. 4. Mitosis, meiosis and cell division, chromosomal structure and organization of chromosomes. 5. Methods of clinical cytogenetics, classical cytogenetics, mitotic metaphase chromosome preparation, karyotype, idiogram. Chromosome banding methods: Q, G, C, R, Ag -banding. Automation of metaphase finding and imaging. An International Systém of Human Cytogenomics Nomenclature (ISCN2016). 6. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in clinical and cancer cytogenetics. 7. Interphase FISH, multicolor FISH (M-FISH), spectral karyotyping (SKY), mBAND. Microarray-CGH. NGS in cytogenetics. 8. Clinical cytogenetics and cytogenomics. Cytogenetics in reproduction medicine. Prenatal and preimplantation cytogenetic diagnosis, detection of common aneuploidies. 9. Mechanisms of chromosome rearrangements, chromosome structural variation, copy number variation (CNVs). 10. The origin and consequences of chromosome pathology, microdeletion and microduplication syndromes, inversion, translocation. Marker chromosomes. 11. Cytogenetic abnormalities in hematological malignancies and solid tumors, chromothripsis. 12. Application of short-term cytogenetics assays for screening of environmental mutagens – CAPL test, micronucleus test, sister chromatid exchanges. 13. The European standard EN ISO 15189:2013 Requirements for quality and competence of medical (cytogenetic) laboratories.
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 % of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics and cytogenomics
Faculty of ScienceSpring 2022
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 8:00–9:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ) || Bi4010 Essential molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 8 fields of study the course is directly associated with, display
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic and cytogenomics techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Learning outcomes
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Genetics, cytogenetics and cytogenomics. Human cytogenetics. 2. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, fluorescent microscopy. 3. Basic techniques of cytogenetic preparation making. 4. Mitosis, meiosis and cell division, chromosomal structure and organization of chromosomes. 5. Methods of clinical cytogenetics, classical cytogenetics, mitotic metaphase chromosome preparation, karyotype, idiogram. Chromosome banding methods: Q, G, C, R, Ag -banding. Automation of metaphase finding and imaging. An International Systém of Human Cytogenomics Nomenclature (ISCN2016). 6. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in clinical and cancer cytogenetics. 7. Interphase FISH, multicolor FISH (M-FISH), spectral karyotyping (SKY), mBAND. Microarray-CGH. NGS in cytogenetics. 8. Clinical cytogenetics and cytogenomics. Cytogenetics in reproduction medicine. Prenatal and preimplantation cytogenetic diagnosis, detection of common aneuploidies. 9. Mechanisms of chromosome rearrangements, chromosome structural variation, copy number variation (CNVs). 10. The origin and consequences of chromosome pathology, microdeletion and microduplication syndromes, inversion, translocation. Marker chromosomes. 11. Cytogenetic abnormalities in hematological malignancies and solid tumors, chromothripsis. 12. Application of short-term cytogenetics assays for screening of environmental mutagens – CAPL test, micronucleus test, sister chromatid exchanges. 13. The European standard EN ISO 15189:2013 Requirements for quality and competence of medical (cytogenetic) laboratories.
- Literature
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 % of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics and cytogenomics
Faculty of ScienceSpring 2021
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 1. 3. to Fri 14. 5. Wed 9:00–10:50 online_B1
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ) || Bi4010 Essential molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic and cytogenomics techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Learning outcomes
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Genetics, cytogenetics and cytogenomics. Human cytogenetics. 2. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, fluorescent microscopy. 3. Basic techniques of cytogenetic preparation making. 4. Mitosis, meiosis and cell division, chromosomal structure and organization of chromosomes. 5. Methods of clinical cytogenetics, classical cytogenetics, mitotic metaphase chromosome preparation, karyotype, idiogram. Chromosome banding methods: Q, G, C, R, Ag -banding. Automation of metaphase finding and imaging. An International Systém of Human Cytogenomics Nomenclature (ISCN2016). 6. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in clinical and cancer cytogenetics. 7. Interphase FISH, multicolor FISH (M-FISH), spectral karyotyping (SKY), mBAND. Microarray-CGH. NGS in cytogenetics. 8. Clinical cytogenetics and cytogenomics. Cytogenetics in reproduction medicine. Prenatal and preimplantation cytogenetic diagnosis, detection of common aneuploidies. 9. Mechanisms of chromosome rearrangements, chromosome structural variation, copy number variation (CNVs). 10. The origin and consequences of chromosome pathology, microdeletion and microduplication syndromes, inversion, translocation. Marker chromosomes. 11. Cytogenetic abnormalities in hematological malignancies and solid tumors, chromothripsis. 12. Application of short-term cytogenetics assays for screening of environmental mutagens – CAPL test, micronucleus test, sister chromatid exchanges. 13. The European standard EN ISO 15189:2013 Requirements for quality and competence of medical (cytogenetic) laboratories.
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 % of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics and cytogenomics
Faculty of ScienceSpring 2020
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 8:00–9:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 7 fields of study the course is directly associated with, display
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic and cytogenomics techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Learning outcomes
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Genetics, cytogenetics and cytogenomics. Human cytogenetics. 2. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, fluorescent microscopy. 3. Basic techniques of cytogenetic preparation making. 4. Mitosis, meiosis and cell division, chromosomal structure and organization of chromosomes. 5. Methods of clinical cytogenetics, classical cytogenetics, mitotic metaphase chromosome preparation, karyotype, idiogram. Chromosome banding methods: Q, G, C, R, Ag -banding. Automation of metaphase finding and imaging. An International Systém of Human Cytogenomics Nomenclature (ISCN2016). 6. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in clinical and cancer cytogenetics. 7. Interphase FISH, multicolor FISH (M-FISH), spectral karyotyping (SKY), mBAND. Microarray-CGH. NGS in cytogenetics. 8. Clinical cytogenetics and cytogenomics. Cytogenetics in reproduction medicine. Prenatal and preimplantation cytogenetic diagnosis, detection of common aneuploidies. 9. Mechanisms of chromosome rearrangements, chromosome structural variation, copy number variation (CNVs). 10. The origin and consequences of chromosome pathology, microdeletion and microduplication syndromes, inversion, translocation. Marker chromosomes. 11. Cytogenetic abnormalities in hematological malignancies and solid tumors, chromothripsis. 12. Application of short-term cytogenetics assays for screening of environmental mutagens – CAPL test, micronucleus test, sister chromatid exchanges. 13. The European standard EN ISO 15189:2013 Requirements for quality and competence of medical (cytogenetic) laboratories.
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 % of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2019
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 18. 2. to Fri 17. 5. Tue 8:00–9:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotype, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin and significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 %of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of Sciencespring 2018
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 8:00–9:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotype, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin and significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 80 %of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2017
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 20. 2. to Mon 22. 5. Tue 8:00–9:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotype, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin and significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 75% of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2016
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Wed 15:00–16:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotype, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin and significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 75% of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2015
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 9:00–10:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotype, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin and significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 75% of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2014
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 13:00–14:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- The goal of this course is to provide general knowledge about the role of chromosomes in human genetics and use of cytogenetic techniques to obtain diagnostic information. At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern molecular cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogenetic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotype, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin and significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- Written tests on computer. The test consists of 50 questions, 75% of correct answers is needed to pass.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2013
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Mon 13:00–14:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- written tests, oral exam
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2012
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 15:00–16:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-EXB)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- written tests, oral exam
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2011
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Timetable
- Thu 16:00–17:50 B11/306
- Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- written tests, oral exam
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2010
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Timetable
- Tue 16:00–17:50 BR3
- Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- written tests, oral exam
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2009
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Timetable
- Wed 15:00–16:50 BR3
- Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods. At the end of course, student should be able to work in the area of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods
- written tests, oral exam
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2008
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Timetable
- Mon 14:00–15:50 Bpg
- Prerequisites
- ( Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics ) && Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods (in Czech)
- Přednáška. Zkouška: ústní.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2007
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Timetable
- Tue 16:00–17:50 BR3
- Prerequisites
- Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics || Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods (in Czech)
- Přednáška. Zkouška: ústní.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2006
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Timetable
- Wed 16:00–17:50 BR1
- Prerequisites
- Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods (in Czech)
- Přednáška. Zkouška: ústní.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2005
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Timetable
- Wed 13:00–14:50 B1,01004
- Prerequisites
- Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. 14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods (in Czech)
- Přednáška. Zkouška: ústní.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2004
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Prerequisites
- Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. 14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods (in Czech)
- Přednáška. Zkouška: ústní.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2003
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Prerequisites
- Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Basic genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. 14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods (in Czech)
- Přednáška. Zkouška: ústní.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of Sciencespring 2012 - acreditation
The information about the term spring 2012 - acreditation is not made public
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- written tests, oral exam
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2011 - only for the accreditation
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Prerequisites
- Bi3060 Basic genetics && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology ))
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- At the end of the course students should be able to: understand and explain basic aspects of human chromosome biology; use modern cytogenetic techniques and methods; work in the laboratories of clinical and cancer human cytogenetics.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- SNUSTAD, D. Peter, Michael J. SIMMONS, Jiřina RELICHOVÁ, Jiří DOŠKAŘ, Jiří FAJKUS, Petr HOŘÍN, Aleš KNOLL, Petr KUGLÍK, Jan ŠMARDA, Jana ŠMARDOVÁ, Renata VESELSKÁ and Boris VYSKOT. Genetika (Principles of Genetics). 1st ed. Brno: Masarykova univerzita, 2009, 894 pp. Učebnice. ISBN 978-80-210-4852-2. URL info
- NUSSBAUM, Robert L., Roderick R. MCINNES, Huntington F. WILLARD, James THOMPSON and Margaret Wilson THOMPSON. Klinická genetika : Thompson & Thompson. Translated by Petr Goetz. Vyd. 1. Praha: Triton, 2004, 426, lix. ISBN 8072544756. info
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Teaching methods
- lectures
- Assessment methods
- written tests, oral exam
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
Bi6270 Cytogenetics
Faculty of ScienceSpring 2008 - for the purpose of the accreditation
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Petr Kuglík, CSc. (lecturer)
- Guaranteed by
- doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc. - Prerequisites
- Ex_3162 Obecná genetika || Imp_9126 Obecná genetika || B1900 Basic genetics || BMB32 Basic genetics || B6730 Genetics || B8470 Genetics || B3060 Basic genetics || Bi3060 Basic genetics || Bi4020 Molecular biology
Requirements of genetics an molecular biology as one of disciplines of the common part of leaving examination of Biology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Course objectives
- Cytogenetics is one of the oldest branches of genetics. The course is targeted at undergraduate students who need a general introduction to cytogenetics. The two main goals are followed: To present to students detailing all aspect of chromosome biology and the practical teaching of modern cytogenetic techniques and methods.
- Syllabus
- 1. History of cytogenetics. Objects, aims and methods of cytogenetics. Instrumental analysis of cytogentic structures. Light microscopy, phase microscopy, darkfield microscopy, fluorescent microscopy. Cytochemical and histochemical methods. Technical equipment of cytogenetics laboratory. 2. Basic techniques of cytogenetic preparation making. Pretreatment, fixation, maceration, staining, closing of preparations. Paraffin technique, squash methods, spreading methods. 3. Cell cycle and DNA content in eukaryotic cells. 4. Mitosis, meiosis and cell division. 5. Interphase nucleus, chromatin and organization of chromosomes. Relation between structure and function of chromatin. 6. Chromosomal shape, karyotyp, idiogram. Banding methods. Q, G, C, R, N-banding. HRT technique. 7. Physical, chemical mutagens and chromosomal damage. 8. Structural chromosomal changes. 9. Origin a significance of chromosomal aberrations. 10. Application of short-term cytogenetics assays for screening of environmental mutagens - chromosomal aberrations, micronucleus test, sister chromatid exchanges. 11. Human cytogenetics. 12. Cytogenetic abnormalities in hematological malignancies and solid tumors. 13. Molecular cytogenetics. Methods and applications of fluorescence in situ hybridization in biological dosimetry and detection of disease-specific chromosomal alterations. Technique of comparative genomic hybridization (CGH). Spectral karyotyping. Array-CGH.14. An International System for Human Cytogenetic Nomenclature (ISCN).
- Literature
- Kuglík, P.: Vybrané kapitoly z cytogenetiky. Skriptum, Masarykova univerzita, Brno, 2000.
- Kuglík, P.: Základy molekulární cytogenetiky člověka. Skriptum - elektronická forma.IS MU, 2006.
- MICHALOVÁ, Kyra. Úvod do lidské cytogenetiky. Vyd. 1. V Brně: Institut pro další vzdělávání pracovníků ve zdravotnictví v Brně, 1999, 172 s. ISBN 8070132817. info
- Assessment methods (in Czech)
- Přednáška. Zkouška: ústní.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Bi6270c Cytogenetics - practice
Bi3060 && Bi4020 || NOW(Bi4020) && NOW(Bi6270) - Bi7250 Medical genetics and genetic counseling
(Ex_3162 || Imp_9126 || B1900 || BMB32 || B6730 || B8470 || B3060 || Bi3060) && (Ex_3065 || Imp_9115 || B3120 || B4030 || B5740 || B6130 || B7940 || B4020 || Bi4020) && (Ex_2315 || BMB41 || B6270 || Bi6270 || NOW(Bi6270)) && !B7250
- Bi6270c Cytogenetics - practice
- Teacher's information
- http://orion.sci.muni.cz/kgmb/student/6270.htm
- Enrolment Statistics (Spring 2025, recent)