Bi4035 Molecular biology - laboratory practice

Faculty of Science
Spring 2019
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
doc. Mgr. Petr Beneš, Ph.D. (seminar tutor)
Mgr. Jarmila Navrátilová, Ph.D. (seminar tutor)
Mgr. Tibor Botka, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Timetable of Seminar Groups
Bi4035/01_1: Mon 18. 2. to Fri 17. 5. Wed 13:00–16:50 D36/216, V. Růžičková
Bi4035/01_2: Mon 18. 2. to Fri 17. 5. Wed 13:00–16:50 D36/216, V. Růžičková
Bi4035/02_1: Mon 18. 2. to Fri 17. 5. Thu 13:00–16:50 D36/216, T. Botka
Bi4035/02_2: Mon 18. 2. to Fri 17. 5. Thu 13:00–16:50 D36/216, T. Botka
Bi4035/03_1: Mon 18. 2. to Fri 17. 5. Wed 9:00–12:50 D36/216, P. Beneš, J. Navrátilová
Bi4035/03_2: Mon 18. 2. to Fri 17. 5. Wed 9:00–12:50 D36/216, P. Beneš, J. Navrátilová
Prerequisites
( C1600 Laboratory in Chemistry || C1080 General Chemistry-lab.course || C1620 General and Inorg.Chem.-lab. || C1650 Fundamentals of Chem.-lab. || C2100 Inorganic Chemistry-lab.course || C2500 General Chemistry-lab.course || C2740 Fundament. of Chem.-lab.course || C2480 Basic Organic Chem. and Bioch. || Bi9410 Structural Biology || SOUHLAS ) && ( Bi4020 Molecular biology || NOW ( Bi4020 Molecular biology )) && ! Bi4020c Essential methods in mol. biol
Znalost molekulární struktury a funkce prokaryotického a eukaryotického genomu. Znalost základních mikrobiologických technik jako kultivace bakterií, stanovení titru bakteriální suspenze. Znalost výpočtů složení chemických roztoků a jejich příprava. Předpokládá se předchozí absolvování základních cvičení z anorganické a organické chemie a mikrobiologie.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 150 student(s).
Current registration and enrolment status: enrolled: 6/150, only registered: 0/150
fields of study / plans the course is directly associated with
there are 8 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to competently analyse the genetic material including chromosome DNA and solve experiments with plasmids originating from the bacteria.
Learning outcomes
Student will be able to:
isolate plasmid DNA of sufficient quality and quantity;
analyze isolated genetic material by basic methods of molecular biology such as restriction endonuclease cleavage and ELFO;
prepare sufficient amount of PCR amplicon of the gene of interest;
create the construct of the plasmid vector and the insert of the gene of interest and perform the transformation of competent cells;
use theoretical knowledge of other methods and principles in subsequent laboratory practice
Syllabus
  • This course include the practical tasks which serve to acquire the basic methods in the molecular biology, primarily, isolation and manipulation with nucleic acids. Preparation, solutions useful for molecular methods: Isolation of plasmid DNA Concentration and purity of DNA Restriction splitting, ELFO Ultracentrifugation
Literature
Teaching methods
Study materials are available in the IS.
Assessment methods
Students would independently manage the entered tasks and correctly interpret the results in laboratory protocols.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2019/Bi4035