VLLM0421c Medical Microbiology I - practice

Faculty of Medicine
Spring 2011
Extent and Intensity
0/3/0. 4 credit(s). Type of Completion: z (credit).
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
RNDr. Radek Horváth, Ph.D. (seminar tutor)
Mgr. Martina Mahelová, Ph.D. (seminar tutor)
Mgr. Barbora Mališová, Ph.D. (seminar tutor)
doc. MUDr. Filip Růžička, Ph.D. (seminar tutor)
Mgr. Petra Šišková, Ph.D. (seminar tutor)
MUDr. Zuzana Vališová (seminar tutor)
doc. MUDr. Vladana Woznicová, Ph.D. (seminar tutor)
MUDr. Ondřej Zahradníček (seminar tutor)
Ing. Magdalena Flasarová (assistant)
MUDr. Ondřej Zahradníček
Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Timetable of Seminar Groups
VLLM0421c/Absolvents: No timetable has been entered into IS.
VLLM0421c/01_02: Mon 15:45–18:15 N02112, O. Zahradníček
VLLM0421c/03_04: Tue 13:30–16:00 N02112, M. Dvořáková Heroldová
VLLM0421c/05_06: Wed 10:15–12:45 N02112, P. Šišková
VLLM0421c/07_08: Mon 7:30–10:00 N02112, O. Zahradníček
VLLM0421c/09_10: Thu 13:30–16:00 N02112, L. Černohorská
VLLM0421c/11_12: Mon 13:00–15:30 N02112, O. Zahradníček
VLLM0421c/13_16: Tue 16:00–18:30 N02112, M. Dvořáková Heroldová
VLLM0421c/14_15: Wed 13:00–15:30 N02112, B. Mališová
VLLM0421c/17_18: Fri 7:30–10:00 N02112, Z. Vališová
VLLM0421c/19_20: Wed 7:30–10:00 N02112, M. Mahelová
VLLM0421c/30_33: No timetable has been entered into IS. O. Zahradníček
VLLM0421c/31_32: No timetable has been entered into IS. O. Zahradníček
VSLC011p Med.Chem.- lecture && VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. )
Students are supposed to have considerable knowledge of anathomy, histology, biology, biochemistry and physiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
Fields of study the course is directly associated with
Course objectives
At the end of this course, students should be able to understand principles of following diagnostic methods in medical microbiology:
microscopy (wet mount, Gram staining, other staining methods)
culture (culture media, theire use, differences between them)
identification of bacteria and yeasts (various methods of biochemical identification, other identification methods)
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
nucleic acid detection in microbiology (only basically)
methods detecting antigens or antibodies, theire use for antibody detection/antigen detection in specimen/antigen analysis of a strain; titers, titer dynamics, detection of IgM/IgA/IgG; precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will understand the basic of medical virology
  • J01 Safety in laboratory, technique of work with loop, wet mount, simple staining. Demostration of pictures of various organisms in simple staining.
  • J02 Gram staining, demonstration of more staining methods. Demostration of pictures of various organisms in Gram staining.
  • J03 Bacterial culture
  • J04 Fysiology of bacteria, idenctification according to biochemical activity and virulence factor determination
  • J05 Microbes and outer influences, decontamination methods
  • J06 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J07 Introduction to serology, agglutination and precipitation, dilution and following titre dynamics
  • J08 Complementfixing test
  • J09 Neutralisatioin (ASO, HIT, VNT)
  • J10 Reactions with labelled components (IMF, RIA, ELISA) including immunoblotting
  • J12 Medical virology I (influenza, respiratory viruses, tick-borne encefalitis)
  • J13 Medical virology II (hepatitis and HIV)
  • J14 Review
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1. vyd. Brno: Neptun, 2001. 247 pp. ISBN 80-902896-2-2. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1. vyd. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003. 495 pp. ISBN 80-902896-6-5. info
  • VOTAVA, Miroslav, Monika HEROLDOVÁ, Petr ONDROVČÍK, Filip RŮŽIČKA, Vladana WOZNICOVÁ and Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie - protokoly k praktickým cvičením (Medical microbiology - protocoles to practical sessions). 1. vyd. Brno: Vydavatelství Neptun, 2004. 94 pp. ISBN 80-902896-7-3. info
  • VOTAVA, Miroslav, Vlastimil OBDRŽÁLEK, Petr ONDROVČÍK, Filip RŮŽIČKA, Ondřej ZAHRADNÍČEK and Vladana WOZNICOVÁ. Lékařská mikrobiologie II. Přehled vyšetřovacích metod v lékařské mikrobiologii (Medical Microbiology II. Survey of Diagnostic Methods in Medical Microbiology). 1. vyd. Brno: Masarykova univerzita. Lékařská fakulta, 2000. 309 pp. ISBN 80-210-2272-8. info
  • VOTAVA, Miroslav and Petr ONDROVČÍK. Vybrané kapitoly z klinické mikrobiologie (Selected chapters from clinical microbiology). 1. vyd. Brno: Masarykova universita, 1998. 90 pp. ISBN 80-210-1805-4. info
  • BEDNÁŘ, Marek. Lékařská mikrobiologie : bakteriologie, virologie, parazitologie. 1. vyd. Praha: Marvil, 1996. 558 s. info
  • GREENWOOD, David. Lékařská mikrobiologie : přehled infekčních onemocnění : patogeneze, imunita, laboratorní diagnostika a epidemiologie. 1. vyd. Praha: Grada Publishing, 1999. 686 s. ISBN 80-7169-365-0. info
  • Medical microbiology : A guide to microbial infections: Pathogenesis, immunity, laboratory diagnosis and control. Edited by David Greenwood - Richard C. B. Slack - John F. Peutherer - Barer M. 17th ed. Edinburgh: Churchill Livingstone, 2007. 738 s. ISBN 978-0-443-10209-7. info
  • MURRAY, Patrick R., Ken S. ROSENTHAL and George S., et al. KOBAYASHI. Medical microbiology. 4th ed. St. Louis: Mosby, 2002. 826 s. ISBN 0-323-01213-2. info
  • MIMS, Cedric A. Medical microbiology. Updated 3rd ed. Edinburgh: Elsevier, 2004. ix, 660. ISBN 0323035752. info
  • Pro české studium jsou základní tituly 1, 2 a 3, ostatní jsou doplňkové. For English study, title No. 8 is basic, plus protocoles on IS MU. Titles No. 9 and 10 are supplementary.
  • Používejte také / Use also: www.medmicro.info.
Teaching methods
practical training in lab
self-study with use of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with following notes:
- two justified absences or absences approved by teacher are allowed; students are obliged to show that they completed the knowledge (in laboratory reports and in their heads)
- more absences than two require a substitution
- not justified absences are not allowed (EXTRA PAYMENT IS REQUIRED FOR EVENTUAL SUBSTITUTION!!!)
(2) complete laboratory report (signature of teacher is not necessary)
(3) succesfully written credit test, eventually more tests; date of all tests will be declared in time.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
Listed among pre-requisites of other courses

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Teacher's information
The course is also listed under the following terms Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019.
  • Enrolment Statistics (Spring 2011, recent)
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