VLLM0421c Medical Microbiology I - practice

Faculty of Medicine
Spring 2017
Extent and Intensity
0/3/0. 4 credit(s). Type of Completion: z (credit).
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
Mgr. Ing. Veronika Lýčková (seminar tutor)
Mgr. Katarína Rebrošová (seminar tutor)
doc. MUDr. Filip Růžička, Ph.D. (seminar tutor)
Mgr. Lukáš Vacek (seminar tutor)
doc. MUDr. Filip Růžička, Ph.D.
Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Timetable of Seminar Groups
VLLM0421c/Uzn: No timetable has been entered into IS., Virtuální seminární skupina pro studenty, kteří mají předmět uznán
VLLM0421c/01_02: Mon 15:45–18:15 N02112, O. Zahradníček
VLLM0421c/03_04: Tue 13:00–15:30 N02112, L. Vacek
VLLM0421c/05_06: Wed 10:15–12:45 N02112, M. Dvořáčková
VLLM0421c/07_08: Mon 13:00–15:30 N02112, O. Zahradníček
VLLM0421c/09_10: Thu 13:45–16:15 N02112, L. Černohorská
VLLM0421c/11_12: Mon 7:30–10:00 N02112, O. Zahradníček
VLLM0421c/13_16: Tue 16:00–18:30 N02112, V. Lýčková
VLLM0421c/14_15: Wed 13:00–15:30 N02112, M. Dvořáková Heroldová
VLLM0421c/17_18: Wed 7:30–10:00 N02112, M. Dvořáková Heroldová
VLLM0421c/41: Fri 10:30–13:00 N02112, L. Vacek
VLLM0421c/42: Wed 17:30–20:00 N02112, O. Zahradníček
( VSBI0222p Biology II-lect. || VLBI0222p Biology II-lect. ) && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. )
Students are supposed to have considerable knowledge of anathomy, histology, biology, biochemistry and physiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
Fields of study the course is directly associated with
Course objectives
At the end of this course, students should be able:
to prepare wet mount, Gram stained preparation, to observe the results in a microscope, to list other staining methods
to list culture media, their use, differences between them, to reinoculate a strain/specimen to a culture medium
to identify bacteria and yeasts by means of various methods of biochemical identification and other identification methods
to describe antibiotic susceptibility/resistance testing methods and to read their results
to describe animal experiment (only basically)
to read results of nucleic acid detection in microbiology (only basically)
to describe methods detecting antigens or antibodies, theire use for antibody detection/antigen detection in specimen/antigen analysis of a strain; to read the results of these methods including titers, titer dynamics, detection of IgM/IgA/IgG; to interprete the results; to describe precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will be able to describe the basic of medical virology
  • J01 Safety in laboratory, technique of work with loop, wet mount. Gram staining, demonstration of more staining methods. Demostration of pictures of various organisms and structures in Gram staining.
  • J02 Bacterial culture
  • J03 Physiology of bacteria, idenctification according to biochemical activity and virulence factor determination
  • J04 Microbes and outer influences, decontamination methods
  • J05 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J06 Introduction to serology, agglutination and precipitation, dilution and measuring titres
  • J07 Following dynamics of titres, complement fixing test, neutralisation
  • J08 Reactions with labelled components (IMF, RIA, ELISA) including immunoblotting
  • J09 Molecular methods in microbiology
  • J10 Medical virology I (influenza, respiratory viruses, tick-borne encefalitis)
  • J11 Medical virology II (hepatitis and HIV))
  • J12 Medical parasitology (basics)
  • J13 Medical mycology (basics)
  • J14 Biofilm infections
  • J15 No practical sessions (only exceptionally individual substitutions)
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1. vyd. Brno: Neptun, 2001. 247 pp. ISBN 80-902896-2-2. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1. vyd. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003. 495 pp. ISBN 80-902896-6-5. info
  • VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody (Medical Microbiology: Laboratory Procedures). 1. vyd. Brno: Neptun, 2010. 495 pp. ISBN 978-80-86850-04-7. info
  • GREENWOOD, David. Lékařská mikrobiologie : přehled infekčních onemocnění : patogeneze, imunita, laboratorní diagnostika a epidemiologie. 1. vyd. Praha: Grada Publishing, 1999. 686 s. ISBN 80-7169-365-0. info
  • RULÍK, Martin, Veronika HOLÁ, Filip RŮŽIČKA, Miroslav VOTAVA, Dana BAUDIŠOVÁ, Jiří GALLO, Simona KAPRÁLOVÁ, Kateřina KOHUŠOVÁ, Dagmar KOUKALOVÁ, Václav KŮDELA, Jiří MIKEŠ, Radko NOVOTNÝ, Martina SIGLOVÁ, Věra TORŠOVÁ and Jiří ZIMÁK. Mikrobiální biofilmy (Microbial Biofilms). 1. vydání. Olomouc: Univerzita Palackého v Olomouci, 2011. 448 pp. Monografie. ISBN 978-80-244-2747-8. info
  • Medical microbiology : a guide to microbial infections : pathogenesis, immunity, laboratory diagnosis and control. Edited by David Greenwood. 18th ed. Edinburgh: Churchill Livingstone, 2012. xvi, 778. ISBN 9780702040900. info
  • MURRAY, Patrick R., Ken S. ROSENTHAL and George S., et al. KOBAYASHI. Medical microbiology. 6th ed. St. Louis: Mosby, 2008. 960 pp. ISBN 0-323-05470-6. info
  • BJARNSHOLT, Thomas, Claus MOSER and Niels HØIBY. Biofilm Infections. : Springer, 2011. ISBN 978-1-4419-6084-9. e-book info
  • Tituly 1, 2, přednášky v IS MU a veškeré materiály z praktik jsou základní studijní materiály ke zkoušce, ostatní jsou doplňkové.
Teaching methods
practical training in lab
self-study with use of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with following notes:
- two justified absences or absences approved by teacher are allowed; students are obliged to show that they completed the knowledge (in laboratory reports and in their heads)
- more absences than two require a substitution
- not justified absences are not allowed
(2) complete laboratory report (signature of teacher is not necessary)
(3) succesfully written final test (usually 10 multiple choice questions, each for one point; 7 points needed)
(4) all ROPOT questionaires completed succesfully; if they are always fulfilled in time (prior to particullar lab session), the student has a bonus of one point for final test
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2018, spring 2019.
  • Enrolment Statistics (Spring 2017, recent)
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