VLLM0421c Medical Microbiology I - practice

Faculty of Medicine
Spring 2013
Extent and Intensity
0/3/0. 4 credit(s). Type of Completion: z (credit).
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
RNDr. Radek Horváth, Ph.D. (seminar tutor)
Mgr. Martina Mahelová, Ph.D. (seminar tutor)
Mgr. Barbora Mališová, Ph.D. (seminar tutor)
Mgr. Tereza Peroutková (seminar tutor)
doc. MUDr. Filip Růžička, Ph.D. (seminar tutor)
MUDr. Alena Siváková (seminar tutor)
Mgr. Petra Šišková, Ph.D. (seminar tutor)
doc. MUDr. Vladana Woznicová, Ph.D. (seminar tutor)
Ing. Magdalena Flasarová (assistant)
doc. MUDr. Filip Růžička, Ph.D.
Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology - Institutions shared with St. Anne's Faculty Hospital - Faculty of Medicine
Timetable of Seminar Groups
VLLM0421c/UZN: No timetable has been entered into IS.
VLLM0421c/0: No timetable has been entered into IS. O. Zahradníček
VLLM0421c/01_02: Mon 15:45–18:15 N02112, A. Siváková
VLLM0421c/03_04: Tue 13:00–15:30 N02112, T. Peroutková
VLLM0421c/05_06: Wed 10:15–12:45 N02112, M. Dvořáková Heroldová
VLLM0421c/07_08: Mon 7:30–10:00 N02112, L. Černohorská
VLLM0421c/09_10: Thu 13:45–16:15 N02112, T. Peroutková
VLLM0421c/11_12: Mon 13:00–15:30 N02112, O. Zahradníček
VLLM0421c/13_16: Tue 16:00–18:30 N02112, M. Dvořáčková
VLLM0421c/14_15: Wed 13:00–15:30 N02112, M. Dvořáková Heroldová
VLLM0421c/17_18: Fri 7:30–10:00 N02112, P. Šišková
VLLM0421c/19_20: Wed 7:30–10:00 N02112, M. Mahelová
VLLM0421c/30_32: No timetable has been entered into IS. O. Zahradníček
VLLM0421c/31_35_36: No timetable has been entered into IS. A. Siváková
VLLM0421c/33_34: No timetable has been entered into IS. O. Zahradníček
VLLM0421c/41: Mon 10:15–12:45 N02112, M. Mahelová
VLLM0421c/42: Fri 10:30–13:00 N02112, P. Šišková
VSLC011p Med. Chem. lecture && VSBI0222p Biology II-lect. && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. )
Students are supposed to have considerable knowledge of anathomy, histology, biology, biochemistry and physiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
Fields of study the course is directly associated with
Course objectives
At the end of this course, students should be able to understand principles of following diagnostic methods in medical microbiology:
microscopy (wet mount, Gram staining, other staining methods)
culture (culture media, theire use, differences between them)
identification of bacteria and yeasts (various methods of biochemical identification, other identification methods)
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
nucleic acid detection in microbiology (only basically)
methods detecting antigens or antibodies, theire use for antibody detection/antigen detection in specimen/antigen analysis of a strain; titers, titer dynamics, detection of IgM/IgA/IgG; precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will understand the basic of medical virology
  • J01 Safety in laboratory, technique of work with loop, wet mount, simple staining. Demostration of pictures of various organisms in simple staining.
  • J02 Gram staining, demonstration of more staining methods. Demostration of pictures of various organisms in Gram staining.
  • J03 Bacterial culture
  • J04 Fysiology of bacteria, idenctification according to biochemical activity and virulence factor determination
  • J05 Microbes and outer influences, decontamination methods
  • J06 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J07 Introduction to serology, agglutination and precipitation, dilution and following titre dynamics
  • J08 Complementfixing test
  • J09 Neutralisatioin (ASO, HIT, VNT)
  • J10 Reactions with labelled components (IMF, RIA, ELISA) including immunoblotting
  • J12 Medical virology I (influenza, respiratory viruses, tick-borne encefalitis)
  • J13 Medical virology II (hepatitis and HIV)
  • J14 Review
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1. vyd. Brno: Neptun, 2001. 247 pp. ISBN 80-902896-2-2. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1. vyd. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003. 495 pp. ISBN 80-902896-6-5. info
  • VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody (Medical Microbiology: Laboratory Procedures). 1. vyd. Brno: Neptun, 2010. 495 pp. ISBN 978-80-86850-04-7. info
  • GREENWOOD, David. Lékařská mikrobiologie : přehled infekčních onemocnění : patogeneze, imunita, laboratorní diagnostika a epidemiologie. 1. vyd. Praha: Grada Publishing, 1999. 686 s. ISBN 80-7169-365-0. info
  • BEDNÁŘ, Marek. Lékařská mikrobiologie : bakteriologie, virologie, parazitologie. 1. vyd. Praha: Marvil, 1996. 558 s. info
  • RULÍK, Martin, Veronika HOLÁ, Filip RŮŽIČKA, Miroslav VOTAVA, Dana BAUDIŠOVÁ, Jiří GALLO, Simona KAPRÁLOVÁ, Kateřina KOHUŠOVÁ, Dagmar KOUKALOVÁ, Václav KŮDELA, Jiří MIKEŠ, Radko NOVOTNÝ, Martina SIGLOVÁ, Věra TORŠOVÁ and Jiří ZIMÁK. Mikrobiální biofilmy (Microbial Biofilms). 1. vydání. Olomouc: Univerzita Palackého v Olomouci, 2011. 448 pp. Monografie. ISBN 978-80-244-2747-8. info
  • Medical microbiology : A guide to microbial infections: Pathogenesis, immunity, laboratory diagnosis and control. Edited by David Greenwood - Richard C. B. Slack - John F. Peutherer - Barer M. 17th ed. Edinburgh: Churchill Livingstone, 2007. 738 s. ISBN 978-0-443-10209-7. info
  • MURRAY, Patrick R., Ken S. ROSENTHAL and George S., et al. KOBAYASHI. Medical microbiology. 6th ed. St. Louis: Mosby, 2008. 960 pp. ISBN 0-323-05470-6. info
  • GOERING, R., ET AL. Mims’ Medical Microbiology, 4th. ed. Edited by Cedric A. Mims. : Mosby, 2007. info
  • BJARNSHOLT, Thomas, Claus MOSER and Niels HØIBY. Biofilm Infections. : Springer, 2011. ISBN 978-1-4419-6084-9. e-book info
  • Pro české studium jsou základní tituly 1, 2 a protokoly z IS MU, ostatní jsou doplňkové. For English study, title No. 7 is essential, plus protocoles on IS MU. Titles No. 8 and 9 are supplementary.
Teaching methods
practical training in lab
self-study with use of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with following notes:
- two justified absences or absences approved by teacher are allowed; students are obliged to show that they completed the knowledge (in laboratory reports and in their heads)
- more absences than two require a substitution
- not justified absences are not allowed (EXTRA PAYMENT IS REQUIRED FOR EVENTUAL SUBSTITUTION!!!)
(2) complete laboratory report (signature of teacher is not necessary)
(3) succesfully written credit test, eventually more tests; date of all tests will be declared in time.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
Listed among pre-requisites of other courses

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Teacher's information
The course is also listed under the following terms Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019.
  • Enrolment Statistics (Spring 2013, recent)
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